Molecular characterization of microbial communities in the rhizosphere soils and roots of diseased and healthy Panax notoginseng

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 城市与区域生态国家重点实验室

	Molecular characterization of microbial communities in the rhizosphere soils and roots of diseased and healthy Panax notoginseng
	Wu, Zhaoxiang; Hao, Zhipeng; Zeng, Yan; Guo, Lanping; Huang, Luqi; Chen, Baodong
刊名	ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
	2015-11
卷号	108 期号:5 页码:1059-1074
关键词	Root-rot disease P. notoginseng Rhizosphere soil Illumina MiSeq Microbial community
英文摘要	Rhizosphere and root-associated microbial communities are known to be related to soil-borne disease and plant health. In the present study, the microbial communities in rhizosphere soils and roots of both healthy and diseased Panax notoginseng were analyzed by high-throughput sequencing of 16S rRNA for bacteria and 18S rRNA internal transcribed spacer for fungi, to reveal the relationship of microbial community structure with plant health status. In total, 5593 bacterial operational taxonomic units (OTUs) and 963 fungal OTUs were identified in rhizosphere soils, while 1794 bacterial and 314 fungal OTUs were identified from root samples respectively. Principal coordinate analysis separated the microbial communities both in the rhizosphere soils and roots of diseased P. notoginseng from healthy plants. Compared to those of healthy P. notoginseng, microbial communities in rhizosphere soils and roots of diseased plants showed a decrease in alpha diversity. By contrast, bacterial community dissimilarity increased and fungal community dissimilarity decreased in rhizosphere soils of diseased plants, while both bacterial and fungal community dissimilarity in roots showed no significant difference between healthy and diseased plants. Redundancy analysis at the phylum level showed that mycorrhizal colonization and soil texture significantly affected microbial community composition in rhizosphere soils, whereas shoot nutrition status had a significant effect on microbial community composition in root samples. Our study provided strong evidence for the hypothesis that microbial diversity could potentially serve as an indicator for disease outbreak of medicinal plants, and supported the ecological significance of microbial communities in maintaining plant healthy and soil fertility.
研究领域[WOS]	Microbiology
WOS记录号	WOS:000362881400005
公开日期	2016-03-08
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/32341]
专题	生态环境研究中心_城市与区域生态国家重点实验室
推荐引用方式 GB/T 7714	Wu, Zhaoxiang,Hao, Zhipeng,Zeng, Yan,et al. Molecular characterization of microbial communities in the rhizosphere soils and roots of diseased and healthy Panax notoginseng[J]. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,2015,108(5):1059-1074.
APA	Wu, Zhaoxiang,Hao, Zhipeng,Zeng, Yan,Guo, Lanping,Huang, Luqi,&Chen, Baodong.(2015).Molecular characterization of microbial communities in the rhizosphere soils and roots of diseased and healthy Panax notoginseng.ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,108(5),1059-1074.
MLA	Wu, Zhaoxiang,et al."Molecular characterization of microbial communities in the rhizosphere soils and roots of diseased and healthy Panax notoginseng".ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 108.5(2015):1059-1074.