A Turnip crinkle virus Isolate Lacking the CP Counter-Defence Protein Gene Providing Protection Against the Wild-Type Strain is Associated with Highly Localized RNA Silencing

	A Turnip crinkle virus Isolate Lacking the CP Counter-Defence Protein Gene Providing Protection Against the Wild-Type Strain is Associated with Highly Localized RNA Silencing
	Chen, Ying-Juan ; Zhu, Feng ; Liu, Jian ; Deng, Xing-Guang ; Pu, Xiao-Jun ; Chen, Li-Juan ; Sun, Tian-Lin ; Bao, Wei-Kai ; Xi, De-Hui ; Lin, Hong-Hui
刊名	JOURNAL OF PHYTOPATHOLOGY
	2014
卷号	162 期号:11-12 页码:758-769
关键词	China cross-protection highly localized RNA silencing Turnip crinkle virus
通讯作者	Lin, HH (reprint author), Sichuan Univ, State Key Lab Hydraul & Mt River Engn, Key Lab Bioresource & Ecoenvironm, Coll Life Sci,Minist Educ, Chengdu 610064, Peoples R China.
产权排序	2
合作状况	其它
英文摘要	Cross-protection has been used successfully and commercially to control a range of virus diseases for which the selection of suitable mild strains of plant viruses is necessary. Turnip crinkle virus (TCV) is highly pathogenic on Arabidopsis plants and its silencing suppressor-defective mutant, TCVCP, can induce highly localized RNA silencing which is differs from that of other protective strains. We found that TCVCP provides some protection against wild-type TCV but lacks complete protection, and the relative locations of the protective virus and challenge virus affect the degree of cross-protection. However, similar cross-protection afforded by TCVCP is not observed in Nicotiana benthamiana plants. As expected, TCVCP pre-infected Arabidopsis plants fail to protect against infection with the unrelated Cucumber mosaic virus, strain Fhy. It appears that cross-protection afforded by TCVCP requires that the challenge virus be very similar in sequence, which is a characteristic of RNA silencing. In order to investigate whether the protection is associated with the highly localized RNA silencing, mutant plants involved in key silencing pathway genes of RNA silencing machinery, including dcl2, dcl4 and triple dcl2/dcl3/dcl4 mutants were used. The results demonstrate that cross-protection afforded by TCVCP is dependent on host RNA silencing, and both DCL2 and DCL4 play important roles in this process.
学科主题	Plant Sciences
收录类别	SCI
语种	英语
公开日期	2016-02-26
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/26494]
专题	成都生物研究所_生态研究
推荐引用方式 GB/T 7714	Chen, Ying-Juan,Zhu, Feng,Liu, Jian,et al. A Turnip crinkle virus Isolate Lacking the CP Counter-Defence Protein Gene Providing Protection Against the Wild-Type Strain is Associated with Highly Localized RNA Silencing[J]. JOURNAL OF PHYTOPATHOLOGY,2014,162(11-12):758-769.
APA	Chen, Ying-Juan.,Zhu, Feng.,Liu, Jian.,Deng, Xing-Guang.,Pu, Xiao-Jun.,...&Lin, Hong-Hui.(2014).A Turnip crinkle virus Isolate Lacking the CP Counter-Defence Protein Gene Providing Protection Against the Wild-Type Strain is Associated with Highly Localized RNA Silencing.JOURNAL OF PHYTOPATHOLOGY,162(11-12),758-769.
MLA	Chen, Ying-Juan,et al."A Turnip crinkle virus Isolate Lacking the CP Counter-Defence Protein Gene Providing Protection Against the Wild-Type Strain is Associated with Highly Localized RNA Silencing".JOURNAL OF PHYTOPATHOLOGY 162.11-12(2014):758-769.