Compact multi-band fluorescent microscope with an electrically tunable lens for autofocusing

CORC > 西安光学精密机械研究所 > 中国科学院西安光学精密机械研究所 > 瞬态光学技术国家重点实验室

	Compact multi-band fluorescent microscope with an electrically tunable lens for autofocusing
	Wang, Zhaojun 1; Lei, Ming1 ; Yao, Baoli1 ; Cai, Yanan 1; Liang, Yansheng 1; Yang, Yanlong1 ; Yang, Xibin 2; Li, Hui 2; Xiong, Daxi 2
刊名	biomedical optics express
	2015-11-01
卷号	6 期号:11 页码:4353-4364
ISSN号	2156-7085
产权排序	1
英文摘要	autofocusing is a routine technique in redressing focus drift that occurs in time-lapse microscopic image acquisition. to date, most automatic microscopes are designed on the distance detection scheme to fulfill the autofocusing operation, which may suffer from the low contrast of the reflected signal due to the refractive index mismatch at the water/glass interface. to achieve high autofocusing speed with minimal motion artifacts, we developed a compact multi-band fluorescent microscope with an electrically tunable lens (etl) device for autofocusing. a modified searching algorithm based on equidistant scanning and curve fitting is proposed, which no longer requires a single-peak focus curve and then efficiently restrains the impact of external disturbance. this technique enables us to achieve an autofocusing time of down to 170 ms and the reproductivity of over 97%. the imaging head of the microscope has dimensions of 12 cm x 12 cm x 6 cm. this portable instrument can easily fit inside standard incubators for real-time imaging of living specimens. (c) 2015 optical society of america
学科主题	biochemical research methods ; optics ; radiology, nuclear medicine & medical imaging
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; optics ; radiology, nuclear medicine & medical imaging
研究领域[WOS]	biochemistry & molecular biology ; optics ; radiology, nuclear medicine & medical imaging
关键词[WOS]	liquid-crystal ; focus
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000366122400015
内容类型	期刊论文
源URL	[http://ir.opt.ac.cn/handle/181661/27661]
专题	西安光学精密机械研究所_瞬态光学技术国家重点实验室
作者单位	1.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Peoples R China 2.Chinese Acad Sci, Suzhou Inst Biomed Engn & Technol, Jiangsu Key Lab Med Opt, Suzhou 215163, Peoples R China
推荐引用方式 GB/T 7714	Wang, Zhaojun,Lei, Ming,Yao, Baoli,et al. Compact multi-band fluorescent microscope with an electrically tunable lens for autofocusing[J]. biomedical optics express,2015,6(11):4353-4364.
APA	Wang, Zhaojun.,Lei, Ming.,Yao, Baoli.,Cai, Yanan.,Liang, Yansheng.,...&Xiong, Daxi.(2015).Compact multi-band fluorescent microscope with an electrically tunable lens for autofocusing.biomedical optics express,6(11),4353-4364.
MLA	Wang, Zhaojun,et al."Compact multi-band fluorescent microscope with an electrically tunable lens for autofocusing".biomedical optics express 6.11(2015):4353-4364.