Biosensor Based on Imaging Ellipsometry for Serotype-Specific Detection of Riemerella Anatipestifer

	Biosensor Based on Imaging Ellipsometry for Serotype-Specific Detection of Riemerella Anatipestifer
	Huang CH; Li JX; Tang Y; Wang CX; Hou CJ; Huo DQ; Chen YY(陈艳艳); Jin G(靳刚)
刊名	MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
	2011
通讯作者邮箱	huodq@cqu.edu.cn ; yychen2006@sinano.ac.cn ; gajin@imech.ac.cn
卷号	31 期号:7 页码:1609-1613
关键词	Biosensor Imaging ellipsometry Riemerella anatipestifer Detection
ISSN号	0928-4931
通讯作者	Jin, G (reprint author), CAS, Inst Mech, Beijing 100190, Peoples R China.
产权排序	[Jin, Gang] CAS, Inst Mech, Beijing 100190, Peoples R China; [Huang, Chenghong; Hou, Changjun; Huo, Danqun] Chongqing Univ, Key Lab Biorheol Sci & Technol, Minist Educ, Ctr Bioengn, Chongqing 400030, Peoples R China; [Huang, Chenghong; Wang, Chunxia; Chen, Yanyan] CAS, Suzhou Inst Nanotech & Nanobion, Suzhou 215123, Peoples R China; [Li, Jixiang; Tang, Yu] Southwest Univ, Chongqing 402460, Peoples R China
中文摘要	In this study, a practicable method for the detection of Riemerella anatipestifer (R. anatipestifer) using biosensor based on imaging ellipsometry (BIE) is described. The method is performed by immobilizing anti-R. anatipestifer egg yolk immunoglubilin (IgY) onto modified chemistry surface to form sensing layer. Antigen captured by sensing layer can then be quantitatively measured through imaging ellipsometry in grayscale format. The results demonstrate that it can detect R. anatipestifer as low as 5.2 x 10(3) CFU/mL. Furthermore, the proposed method can realize specific discrimination for multiple serotypes of 1,2, 4, and 14 of R. anatipestifer. It has the advantages of rapid, simple, high sensitivity and low cost. (C) 2011 Elsevier B.V. All rights reserved.
分类号	一类
类目[WOS]	Materials Science, Biomaterials
研究领域[WOS]	Materials Science
关键词[WOS]	PASTEURELLA-ANATIPESTIFER ; ESCHERICHIA-COLI ; IMMUNOSENSOR ; DUCKS ; VISUALIZATION ; INFECTION
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000295953900051
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/58582]
专题	力学研究所_国家微重力实验室
推荐引用方式 GB/T 7714	Huang CH,Li JX,Tang Y,et al. Biosensor Based on Imaging Ellipsometry for Serotype-Specific Detection of Riemerella Anatipestifer[J]. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,2011,31(7):1609-1613.
APA	Huang CH.,Li JX.,Tang Y.,Wang CX.,Hou CJ.,...&靳刚.(2011).Biosensor Based on Imaging Ellipsometry for Serotype-Specific Detection of Riemerella Anatipestifer.MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,31(7),1609-1613.
MLA	Huang CH,et al."Biosensor Based on Imaging Ellipsometry for Serotype-Specific Detection of Riemerella Anatipestifer".MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 31.7(2011):1609-1613.