A CCL21 chemokine of tongue sole (Cynoglossus semilaevis) promotes host resistance against bacterial infection

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	A CCL21 chemokine of tongue sole (Cynoglossus semilaevis) promotes host resistance against bacterial infection
	Wang, Ming-qing 1,2; Chi, Heng 1,2; Li, Mo-fei1,2
刊名	FISH & SHELLFISH IMMUNOLOGY
	2015-11-01
卷号	47 期号:1 页码:461-469
关键词	Cynoglossus semilaevis Chemokine Chemotaxis Antibacterial
英文摘要	Chemokines are a large family of chemotactic cytokines. Based on the arrangement of the first two cysteine residues, chemokines are divided into four groups, one of which is the CC chemoldne group. In this study, we characterized a CC chemokine, CsCCL21, from half-smooth tongue sole (cynoglossus semilaevis), and analyzed its activity. CsCCL21 contains two conserved N-terminal cysteine residues in a NCCL motif and is phylogenetically related to the CCL19/21/25 subgroup of CC chemokines. CsCCL21 was constitutively expressed in nine tissues and significantly upregulated by bacterial and viral infection. The recombinant CsCCL21 (rCsCCL21) induced migration of peripheral blood leukocytes. When the two conserved cysteine residues in the NCCL motif were mutated, the chemotactic activity of rCsCCL21 was abolished. rCsCCL21 enhanced the resistance of tongue sole against bacterial infection, but the mutant protein with NCCL mutation lacked this antibacterial effect. Taken together, these results suggest that CsCCL21 is a functional CC chemokine with the ability to recruit leukocytes and is involved in antibacterial immunity in a manner that requires the conserved NCCL motif. (c) 2015 Elsevier Ltd. All rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000365053300053
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/82324]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Wang, Ming-qing,Chi, Heng,Li, Mo-fei. A CCL21 chemokine of tongue sole (Cynoglossus semilaevis) promotes host resistance against bacterial infection[J]. FISH & SHELLFISH IMMUNOLOGY,2015,47(1):461-469.
APA	Wang, Ming-qing,Chi, Heng,&Li, Mo-fei.(2015).A CCL21 chemokine of tongue sole (Cynoglossus semilaevis) promotes host resistance against bacterial infection.FISH & SHELLFISH IMMUNOLOGY,47(1),461-469.
MLA	Wang, Ming-qing,et al."A CCL21 chemokine of tongue sole (Cynoglossus semilaevis) promotes host resistance against bacterial infection".FISH & SHELLFISH IMMUNOLOGY 47.1(2015):461-469.