TLR7 is required for optimal immune defense against bacterial infection in tongue sole (Cynoglossus semilaevis)

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	TLR7 is required for optimal immune defense against bacterial infection in tongue sole (Cynoglossus semilaevis)
	Li, Xue-Peng1,2,3 ; Sun, Li 1,3
刊名	FISH & SHELLFISH IMMUNOLOGY
	2015-11-01
卷号	47 期号:1 页码:93-99
关键词	Toll-like receptor Cynoglossus semilaevis R848 Chloroquine Innate immunity Antibacterial
英文摘要	In mammals as well as in teleost, toll-like receptor 7 (TLR7) is known to be involved in antiviral immunity by recognizing viral RNA. However, the antibacterial potential of fish TLR7 is unclear. In this study, we analyzed the TLR7 of tongue sole (Cynoglossus semilaevis), CsTLR7, and examined its potential involvement in antibacterial immunity. CsTLR7 is composed of 1052 amino acid residues and shares 64.0%-75.9% overall sequence identities with known teleost TLR7. CsTLR7 possesses a toll/interleukin-1 receptor domain and six leucine-rich repeats. Constitutive expression of CsTLR7 occurred in relatively high levels in kidney, spleen and liver. Bacterial infection upregulated CsTLR7 expression, whereas viral infection downregulated CsTLR7 expression. Knockdown of CsTLR7 significantly enhanced bacterial dissemination in the tissues of tongue sole. Treatment of tongue sole with the imidazoquinoline compound R848 (TLR7 activator) and the endosomal acidification inhibitor chloroquine (TLR7 inhibitor) caused enhanced and reduced resistance against bacterial infection respectively. These results indicate that CsTLR7 plays an essential role in the antibacterial immunity of tongue sole. (C) 2015 Elsevier Ltd. All rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000365053300011
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/82320]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qingdao Natl Oceanog Lab, Funct Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Li, Xue-Peng,Sun, Li. TLR7 is required for optimal immune defense against bacterial infection in tongue sole (Cynoglossus semilaevis)[J]. FISH & SHELLFISH IMMUNOLOGY,2015,47(1):93-99.
APA	Li, Xue-Peng,&Sun, Li.(2015).TLR7 is required for optimal immune defense against bacterial infection in tongue sole (Cynoglossus semilaevis).FISH & SHELLFISH IMMUNOLOGY,47(1),93-99.
MLA	Li, Xue-Peng,et al."TLR7 is required for optimal immune defense against bacterial infection in tongue sole (Cynoglossus semilaevis)".FISH & SHELLFISH IMMUNOLOGY 47.1(2015):93-99.