Sensitivity map of laser tweezers Raman spectroscopy for single-cell analysis of colorectal cancer

CORC > 上海光学精密机械研究所 > 中国科学院上海光学精密机械研究所 > 量子光学重点实验室

	Sensitivity map of laser tweezers Raman spectroscopy for single-cell analysis of colorectal cancer
	Zheng Feng ; Qin Yejun ; Chen Kun
刊名	j. biomed. opt.
	2007
卷号	12 期号:3 页码:34002
关键词	laser tweezers Raman spectroscopy colorectal cancer artificial neural network sensitivity map
ISSN号	1083-3668
中文摘要	raman spectroscopy on single, living epithelial cells captured in a laser trap is shown to have diagnostic power over colorectal cancer. this new single-cell technology comprises three major components: primary culture processing of human tissue samples to produce single-cell suspensions, raman detection on singly trapped cells, and diagnoses of the cells by artificial neural network classifications. it is compared with dna flow cytometry for similarities and differences. its advantages over tissue raman spectroscopy are also discussed. in the actual construction of a diagnostic model for colorectal cancer, real patient data were taken to generate a training set of 320 raman spectra and, a test set of 80. by incorporating outlier corrections to a conventional binary neural classifier, our network accomplished significantly better predictions than logistic regressions, with sensitivity improved from 77.5% to 86.3% and specificity improved from 81.3% to 86.3% for the training set and moderate improvements for the test set. most important, the network approach enables a sensitivity map analysis to quantitate the relevance of each raman band to the normal-to-cancer transform at the cell level. our technique has direct clinic applications for diagnosing cancers and basic science potential in the study of cell dynamics of carcinogenesis. (c) 2007 society of photo-optical instrumentation engineers.
学科主题	光学;量子光学
语种	英语
公开日期	2009-09-18
内容类型	期刊论文
源URL	[http://ir.siom.ac.cn/handle/181231/1101]
专题	上海光学精密机械研究所_量子光学重点实验室
推荐引用方式 GB/T 7714	Zheng Feng,Qin Yejun,Chen Kun. Sensitivity map of laser tweezers Raman spectroscopy for single-cell analysis of colorectal cancer[J]. j. biomed. opt.,2007,12(3):34002.
APA	Zheng Feng,Qin Yejun,&Chen Kun.(2007).Sensitivity map of laser tweezers Raman spectroscopy for single-cell analysis of colorectal cancer.j. biomed. opt.,12(3),34002.
MLA	Zheng Feng,et al."Sensitivity map of laser tweezers Raman spectroscopy for single-cell analysis of colorectal cancer".j. biomed. opt. 12.3(2007):34002.