Analysis of telomerase activity based on a spired DNA tetrahedron TS primer

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Analysis of telomerase activity based on a spired DNA tetrahedron TS primer
	Li, Y; Wen, YL; Wang, LL; Liang, W; Xu, L; Ren, SZ; Zou, ZY; Zuo, XL; Fan, CH; Huang, Q
刊名	BIOSENSORS & BIOELECTRONICS
	2015
卷号	67 页码:364—369
关键词	SENSITIVE DETECTION CANCER-CELLS LABEL-FREE SEQUENCE ASSAY BIOSENSOR BLADDER SENSOR
英文摘要	The development of sensitive telomerase biosensors is hindered by the restricted accessibility of telomere strand (TS) primer and the limited enzyme reaction space, which is mainly confined by the vertical distance. In this work, we designed an electrochemical telomerase biosensor based on a spired DNA tetrahedron TS primer (sus). By adding a rigid dsDNA spire onto the top of the DNA tetrahedron, we successfully regulated the distance between the TS primer and the surface, and thus greatly facilitated the telomerase elongation on surface. The signal-to-noise ratio was 2 times higher than TSP without the spire structure. The limit of detection was calculated to be lower than 10 HeLa cells, which is at least 2 magnitudes lower than other surface extension-based electrochemical telomerase sensors without amplification. The practicability of STTS sensor was also demonstrated by analysing various other cell lines including cancer cells, stem cells of high telomerase activity and somatic cells of low telomerase activity. (C) 2014 Elsevier B.V. All rights reserved.
收录类别	SCI
语种	英语
公开日期	2015-12-24
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/24393]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Li, Y,Wen, YL,Wang, LL,et al. Analysis of telomerase activity based on a spired DNA tetrahedron TS primer[J]. BIOSENSORS & BIOELECTRONICS,2015,67:364—369.
APA	Li, Y.,Wen, YL.,Wang, LL.,Liang, W.,Xu, L.,...&Jia, NQ.(2015).Analysis of telomerase activity based on a spired DNA tetrahedron TS primer.BIOSENSORS & BIOELECTRONICS,67,364—369.
MLA	Li, Y,et al."Analysis of telomerase activity based on a spired DNA tetrahedron TS primer".BIOSENSORS & BIOELECTRONICS 67(2015):364—369.