毛细管电泳法以其分离效率高、耗时短、样品用量少、灵敏度高以及易于实现自动化等诸多特点,在筛选酶抑制剂研究中有着不可替代的优势。本文综述了毛细管电泳法在酶抑制剂筛选中的应用。主要介绍了应用最广泛的离线分析(Off-line assay)和在线分析(In-line assay),即柱前酶反应(Pre-capillary enzyme reaction)和在柱酶分析(In-capillary enzyme reaction)。并对该领域的未来发展进行了展望。

Capillary electrophoresis plays an irreplaceable role in the field of enzyme inhibitors screening with unique features of high-efficiency separation, short analysis time, minimal sample consumption, high sensitivity, ease to realize automatization etc. This review gives an overview of the applications of capillary electrophoresis in the field of enzyme inhibitors screening, and summarizes both the widely used off-line (pre-capillary) enzyme assay and on-line (in-capillary) enzyme assay. Finally, some future trends are briefly addressed.