Optimization strategy for purification of a peptide from complex mixtures by reversed-phase liquid chromatography with a back-flushing technique

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	Optimization strategy for purification of a peptide from complex mixtures by reversed-phase liquid chromatography with a back-flushing technique
	Dong, Li Li; Huang, Jun Xiong
刊名	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
	2006
卷号	29 期号:20 页码:2915-2929
关键词	peptide separation tetrahydrofuran preparative chromatography displacement effect back-flushing
英文摘要	An optimization strategy for the separation and purification of a peptide from crude material by reversed-phase liquid chromatography was developed. First, in the separation step, the tetrahydrofuran, instead of normal organic modifiers in the mobile phase, could improve the separation of the target peptide from its impurities; hence, the mass loading could be increased in each run. Furthermore, a back-flushing technique was introduced to elute the strongly retained impurities rapidly and economically. A raw mixture of 10 mg was purified on an analytical column by an overloading operation in one run, using a mobile phase consisting of tetrahydrofuran-water ( 30: 70, v/v) + 0.1% TFA at a flow-rate of 1.0 mL/min and room temperature. The purity of the product was increased from 50% to 98% with recovery over 80%. The back-flushing step was very crucial to regenerate the system for the next run in the sense of both cost and time.
WOS记录号	WOS:000241582000003
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/22902]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Dong, Li Li,Huang, Jun Xiong. Optimization strategy for purification of a peptide from complex mixtures by reversed-phase liquid chromatography with a back-flushing technique[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2006,29(20):2915-2929.
APA	Dong, Li Li,&Huang, Jun Xiong.(2006).Optimization strategy for purification of a peptide from complex mixtures by reversed-phase liquid chromatography with a back-flushing technique.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,29(20),2915-2929.
MLA	Dong, Li Li,et al."Optimization strategy for purification of a peptide from complex mixtures by reversed-phase liquid chromatography with a back-flushing technique".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 29.20(2006):2915-2929.