Improved ethanol fermentation by heterologous endoinulinase and inherent invertase from inulin by Saccharomyces cerevisiae
Yuan, Bo1,3; Wang, Shi-An1,2; Li, Fu-Li1,2
刊名BIORESOURCE TECHNOLOGY
2013-07-01
卷号139页码:402-405
关键词Endoinulinase Biofuel Invertase Inulin Yeast
英文摘要It is hypothesized that introduction of an endoinulinase gene into Saccharomyces cerevisiae will improve its inulin utilization and ethanol fermentation through collaboration between the heterologous endoinulinase and the inherent invertase SUC2. The aim of this work was to test the hypothesis by introducing the endoinulinase gene inuA from Aspergillus niger into S. cerevisiae. The results showed that heterologous inuA expressed in S. cerevisiae selectively digested long chains of inulin into short fructooligosaccharides and parts of these fructooligosaccharides could be efficiently utilized by the yeast. This study demonstrated that collaboration between heterologous endoinulinase and inherent invertase improved inulin degradation and ethanol fermentation in S. cerevisiae. (c) 2013 Elsevier Ltd. All rights reserved.
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
研究领域[WOS]Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
关键词[WOS]SIMULTANEOUS SACCHARIFICATION ; JERUSALEM-ARTICHOKE ; HYDROLYSIS ; BIOETHANOL
收录类别SCI
语种英语
WOS记录号WOS:000321163100059
公开日期2015-12-24
内容类型期刊论文
源URL[http://ir.qibebt.ac.cn/handle/337004/6016]  
专题青岛生物能源与过程研究所_微生物资源团队
作者单位1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Peoples R China
2.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
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Yuan, Bo,Wang, Shi-An,Li, Fu-Li. Improved ethanol fermentation by heterologous endoinulinase and inherent invertase from inulin by Saccharomyces cerevisiae[J]. BIORESOURCE TECHNOLOGY,2013,139:402-405.
APA Yuan, Bo,Wang, Shi-An,&Li, Fu-Li.(2013).Improved ethanol fermentation by heterologous endoinulinase and inherent invertase from inulin by Saccharomyces cerevisiae.BIORESOURCE TECHNOLOGY,139,402-405.
MLA Yuan, Bo,et al."Improved ethanol fermentation by heterologous endoinulinase and inherent invertase from inulin by Saccharomyces cerevisiae".BIORESOURCE TECHNOLOGY 139(2013):402-405.
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