Responses of Nannochloropsis oceanica IMET1 to Long-Term Nitrogen Starvation and Recovery

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 单细胞中心

	Responses of Nannochloropsis oceanica IMET1 to Long-Term Nitrogen Starvation and Recovery
	Dong, Hong-Po 1,2; Williams, Ernest 2; Wang, Da-zhi 3; Xie, Zhang-Xian 3; Hsia, Ru-ching 4; Jenck, Alizee 5; Halden, Rolf 5; Li, Jing 6; Chen, Feng 2; Place, Allen R.2
刊名	PLANT PHYSIOLOGY
	2013-06-01
卷号	162 期号:2 页码:1110-1126
英文摘要	The Nannochloropsis genus contains oleaginous microalgae that have served as model systems for developing renewable biodiesel. Recent genomic and transcriptomic studies on Nannochloropsis species have provided insights into the regulation of lipid production in response to nitrogen stress. Previous studies have focused on the responses of Nannochloropsis species to short-term nitrogen stress, but the effect of long-term nitrogen deprivation remains largely unknown. In this study, physiological and proteomic approaches were combined to understand the mechanisms by which Nannochloropsis oceanica IMET1 is able to endure long-term nitrate deprivation and its ability to recover homeostasis when nitrogen is amended. Changes of the proteome during chronic nitrogen starvation espoused the physiological changes observed, and there was a general trend toward recycling nitrogen and storage of lipids. This was evidenced by a global down-regulation of protein expression, a retained expression of proteins involved in glycolysis and the synthesis of fatty acids, as well as an up-regulation of enzymes used in nitrogen scavenging and protein turnover. Also, lipid accumulation and autophagy of plastids may play a key role in maintaining cell vitality. Following the addition of nitrogen, there were proteomic changes and metabolic changes observed within 24 h, which resulted in a return of the culture to steady state within 4 d. These results demonstrate the ability of N. oceanica IMET1 to recover from long periods of nitrate deprivation without apparent detriment to the culture and provide proteomic markers for genetic modification.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Plant Sciences
研究领域[WOS]	Plant Sciences
关键词[WOS]	CHLAMYDOMONAS-REINHARDTII ; LIPID-ACCUMULATION ; BIOFUEL PRODUCTION ; GROWTH ; METABOLISM ; MICROALGAE ; SEAWATER ; ALGAE ; PURIFICATION ; DEPRIVATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000319819900044
公开日期	2015-12-24
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/6699]
专题	青岛生物能源与过程研究所_单细胞中心
作者单位	1.Jinan Univ, Res Ctr Harmful Algae & Marine Biol, Guangzhou 510632, Guangdong, Peoples R China 2.Univ Maryland, Inst Marine & Environm Technol, Ctr Environm Sci, Baltimore, MD 21202 USA 3.Xiamen Univ, State Key Lab Marine Environm Sci, Xiamen 361005, Peoples R China 4.Univ Maryland, Sch Dent, Dept Microbial Pathogenesis, Baltimore, MD 21202 USA 5.Arizona State Univ, Ctr Environm Secur, Biodesign Inst, Secur & Def Syst Initiat, Tempe, AZ 85287 USA 6.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Genome Ctr, CAS Key Lab Biofuels,Shandong Key Lab Energy Gene, Qingdao 266101, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Dong, Hong-Po,Williams, Ernest,Wang, Da-zhi,et al. Responses of Nannochloropsis oceanica IMET1 to Long-Term Nitrogen Starvation and Recovery[J]. PLANT PHYSIOLOGY,2013,162(2):1110-1126.
APA	Dong, Hong-Po.,Williams, Ernest.,Wang, Da-zhi.,Xie, Zhang-Xian.,Hsia, Ru-ching.,...&Place, Allen R..(2013).Responses of Nannochloropsis oceanica IMET1 to Long-Term Nitrogen Starvation and Recovery.PLANT PHYSIOLOGY,162(2),1110-1126.
MLA	Dong, Hong-Po,et al."Responses of Nannochloropsis oceanica IMET1 to Long-Term Nitrogen Starvation and Recovery".PLANT PHYSIOLOGY 162.2(2013):1110-1126.