Cellulosome stoichiometry in Clostridium cellulolyticum is regulated by selective RNA processing and stabilization

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	Cellulosome stoichiometry in Clostridium cellulolyticum is regulated by selective RNA processing and stabilization
	Xu, Chenggang 1,2,3; Huang, Ranran 1,2,3,4; Teng, Lin 1,2,3,4; Jing, Xiaoyan 1,2,3; Hu, Jianqiang 1,2,3,4; Cui, Guzhen 2,3; Wang, Yilin 1,2,3,4; Cui, Qiu 2,3; Xu, Jian1,2,3
刊名	NATURE COMMUNICATIONS
	2015-04-01
卷号	6
英文摘要	The mechanism, physiological relevance and evolutionary implication of selective RNA processing and stabilization (SRPS) remain elusive. Here we report the genome-wide maps of transcriptional start sites (TSs) and post-transcriptional processed sites (PSs) for Clostridium cellulolyticum. The PS-associated genes are preferably associated with subunits of heteromultimeric protein complexes, and the intergenic PSs (iPSs) are enriched in operons exhibiting highly skewed transcript-abundance landscape. Stem-loop structures associated with those iPSs located at 3' termini of highly transcribed genes exhibit folding free energy negatively correlated with transcript-abundance ratio of flanking genes. In the cellulosome-encoding cip-cel operon, iPSs and stem-loops precisely regulate structure and abundance of the subunit-encoding transcripts processed from a primary polycistronic RNA, quantitatively specifying cellulosome stoichiometry. Moreover, cellulosome evolution is shaped by the number, position and biophysical nature of TSs, iPSs and stem-loops. Our findings unveil a genome-wide RNA-encoded strategy controlling in vivo stoichiometry of protein complexes.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	RELATIVE GENE-EXPRESSION ; MESSENGER-RNA ; ESCHERICHIA-COLI ; BACILLUS-SUBTILIS ; PHOTOSYNTHESIS GENES ; DIFFERENTIAL DECAY ; OPERON ; DEGRADATION ; TRANSCRIPTION ; STABILITY
收录类别	SCI
语种	英语
WOS记录号	WOS:000353704100008
公开日期	2015-12-24
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/6444]
专题	青岛生物能源与过程研究所_单细胞中心
作者单位	1.Chinese Acad Sci, Single Cell Ctr, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Shandong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Shandong, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xu, Chenggang,Huang, Ranran,Teng, Lin,et al. Cellulosome stoichiometry in Clostridium cellulolyticum is regulated by selective RNA processing and stabilization[J]. NATURE COMMUNICATIONS,2015,6.
APA	Xu, Chenggang.,Huang, Ranran.,Teng, Lin.,Jing, Xiaoyan.,Hu, Jianqiang.,...&Xu, Jian.(2015).Cellulosome stoichiometry in Clostridium cellulolyticum is regulated by selective RNA processing and stabilization.NATURE COMMUNICATIONS,6.
MLA	Xu, Chenggang,et al."Cellulosome stoichiometry in Clostridium cellulolyticum is regulated by selective RNA processing and stabilization".NATURE COMMUNICATIONS 6(2015).