Ultrastructure and Composition of the Nannochloropsis gaditana Cell Wall

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	Ultrastructure and Composition of the Nannochloropsis gaditana Cell Wall
	Scholz, Matthew J.1; Weiss, Taylor L.2; Jinkerson, Robert E.1; Jing, Jia 3,4; Roth, Robyn 5; Goodenough, Ursula 2; Posewitz, Matthew C.1; Gerken, Henri G.6
刊名	EUKARYOTIC CELL
	2014-11-01
卷号	13 期号:11 页码:1450-1464
英文摘要	Marine algae of the genus Nannochloropsis are promising producers of biofuel precursors and nutraceuticals and are also harvested commercially for aquaculture feed. We have used quick-freeze, deep-etch electron microscopy, Fourier transform infrared spectroscopy, and carbohydrate analyses to characterize the architecture of the Nannochloropsis gaditana (strain CCMP 526) cell wall, whose recalcitrance presents a significant barrier to biocommodity extraction. The data indicate a bilayer structure consisting of a cellulosic inner wall (similar to 75% of the mass balance) protected by an outer hydrophobic algaenan layer. Cellulase treatment of walls purified after cell lysis generates highly enriched algaenan preparations without using the harsh chemical treatments typically used in algaenan isolation and characterization. Nannochloropsis algaenan was determined to comprise long, straight-chain, saturated aliphatics with ether cross-links, which closely resembles the cutan of vascular plants. Chemical identification of >85% of the isolated cell wall mass is detailed, and genome analysis is used to identify candidate biosynthetic enzymes.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Microbiology
研究领域[WOS]	Microbiology
关键词[WOS]	HAEMATOCOCCUS-PLUVIALIS CHLOROPHYCEAE ; CHLAMYDOMONAS-MONOICA CHLOROPHYTA ; PLANT CELLULOSE SYNTHASE ; FRESH-WATER ALGAE ; BRAUNII RACE B ; SCENEDESMUS CHLOROPHYCEAE ; BOTRYOCOCCUS-BRAUNII ; EXTRACELLULAR-MATRIX ; TRICHODERMA-REESEI ; MARINE MICROALGAE
收录类别	SCI
语种	英语
WOS记录号	WOS:000344646600011
公开日期	2015-12-24
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/6318]
专题	青岛生物能源与过程研究所_单细胞中心
作者单位	1.Colorado Sch Mines, Dept Chem & Geochem, Golden, CO 80401 USA 2.Washington Univ, Dept Biol, St Louis, MO 63130 USA 3.Chinese Acad Sci, CAS Key Lab Biofuels, Shandong Key Lab Energy Genet, Qingdao, Shandong, Peoples R China 4.Chinese Acad Sci, BioEnergy Genome Ctr, Qindao Inst BioEnergy & Bioproc Technol, Qingdao, Shandong, Peoples R China 5.Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA 6.Arizona State Univ, Arizona Ctr Algal Technol & Innovat, Mesa, AZ 85212 USA
推荐引用方式 GB/T 7714	Scholz, Matthew J.,Weiss, Taylor L.,Jinkerson, Robert E.,et al. Ultrastructure and Composition of the Nannochloropsis gaditana Cell Wall[J]. EUKARYOTIC CELL,2014,13(11):1450-1464.
APA	Scholz, Matthew J..,Weiss, Taylor L..,Jinkerson, Robert E..,Jing, Jia.,Roth, Robyn.,...&Gerken, Henri G..(2014).Ultrastructure and Composition of the Nannochloropsis gaditana Cell Wall.EUKARYOTIC CELL,13(11),1450-1464.
MLA	Scholz, Matthew J.,et al."Ultrastructure and Composition of the Nannochloropsis gaditana Cell Wall".EUKARYOTIC CELL 13.11(2014):1450-1464.