Simultaneous accumulation of neutral lipids and biomass in Nannochloropsis oceanica IMET1 under high light intensity and nitrogen replete conditions | |
Xiao, Yan1,2; Zhang, Jingtao1,2; Cui, Jiatao1,2; Yao, Xingzhe1,2,4; Sun, Zhijie1,2; Feng, Yingang1,2,3; Cui, Qiu1,2,3 | |
刊名 | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS |
2015-09-01 | |
卷号 | 11页码:55-62 |
关键词 | Nannochloropsis Neutral lipid Biodiesel High light intensity Nitrogen repletion |
英文摘要 | The difficulty in simultaneously obtaining a high production of neutral lipids and biomass in the oleaginous microalga Nannochloropsis is an attractive challenge that needs to be addressed. To overcome this challenge and unveil the underlying metabolic characteristics, the model oleaginous microalga Nannochloropsis oceanica IMET1, under high light intensity and nitrogen-replete conditions, was prepared via steady-state continuous culture in the present study. The simultaneous accumulation of lipids, particularly neutral lipids, and biomass in N. oceanica IMET1 was triggered at a high light intensity (331.2 mu mol photons m(-2) s(-1)) in the light saturation region accompanied by a sufficient nitrogen supply (2000 mu mol L-1). Moreover, high amounts of valuable fatty acids (arachidonic acid and eicosapentaenoic acid) were also produced. The distribution of fatty acids and intracellular soluble metabolites, the membrane lipid composition and the photosynthetic pathway were associated with the concurrent accumulation of biomass and TAGs in N. oceanica IMET1. The common and different metabolic responses between high-light and nitrogen-depletion strategies for the accumulation of neutral lipids were also discussed. These results provide detailed metabolic clues for the genetic and metabolic engineering of Nannochloropsis for biofuel production. (C) 2015 Elsevier B.V. All rights reserved. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Biotechnology & Applied Microbiology |
研究领域[WOS] | Biotechnology & Applied Microbiology |
关键词[WOS] | GENOME SEQUENCE ; FATTY-ACIDS ; MICROALGAE ; BIODIESEL ; OIL ; TRIACYLGLYCEROL ; STARVATION ; GROWTH ; EUSTIGMATOPHYCEAE ; PHOTOSYNTHESIS |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000363046900008 |
公开日期 | 2015-12-24 |
内容类型 | 期刊论文 |
源URL | [http://ir.qibebt.ac.cn/handle/337004/6697] |
专题 | 青岛生物能源与过程研究所_代谢物组学团队 |
作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Lab Single Cell Oil, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China 4.Chinese Acad Sci, Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Xiao, Yan,Zhang, Jingtao,Cui, Jiatao,et al. Simultaneous accumulation of neutral lipids and biomass in Nannochloropsis oceanica IMET1 under high light intensity and nitrogen replete conditions[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2015,11:55-62. |
APA | Xiao, Yan.,Zhang, Jingtao.,Cui, Jiatao.,Yao, Xingzhe.,Sun, Zhijie.,...&Cui, Qiu.(2015).Simultaneous accumulation of neutral lipids and biomass in Nannochloropsis oceanica IMET1 under high light intensity and nitrogen replete conditions.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,11,55-62. |
MLA | Xiao, Yan,et al."Simultaneous accumulation of neutral lipids and biomass in Nannochloropsis oceanica IMET1 under high light intensity and nitrogen replete conditions".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 11(2015):55-62. |
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